3-Day Hands-On Workshop on Cell Culture Techniques
This workshop introduces participants to fundamental cell culture practices, focusing on aseptic handling, cell maintenance, viability assessment, and contamination awareness. Designed for beginners, it emphasizes safety by using non-pathogenic cell lines or plant cells.
Day 1: Aseptic Techniques & Cell Observation
Concepts Covered
Introduction to cell culture and its applications
Principles of aseptic techniques and laboratory safety
Common sources of contamination and prevention strategies
Hands-On Training
Proper use of laminar airflow / clean bench
Media preparation and sterilization basics
Handling of culture ware and pipetting techniques
Microscopic identification of cells
Cell morphology
Adherent vs suspension cells (or plant cell types)
Skills Gained
Sterile working practices
Media handling and storage
Basic cell observation and documentation
Day 2: Cell Seeding & Viability Assessment
Concepts Covered
Cell growth requirements and culture conditions
Cell attachment and proliferation basics
Importance of viability testing
Hands-On Training
Cell seeding using
Safe, non-pathogenic mammalian cell lines or
Plant cell cultures
Maintenance of cultures under controlled conditions
Cell viability assays
Trypan Blue exclusion test or
Methylene Blue staining
Data Interpretation
Live vs dead cell differentiation
Calculation of cell viability percentage
Recording growth observations
Day 3: Cell Counting, Contamination & Culture Analysis
Concepts Covered
Principles of cell quantification
Types of contamination: bacterial, fungal, yeast, mycoplasma (visual identification)
Best practices for culture monitoring
Hands-On Training
Cell counting using a haemocytometer
Estimation of cell density and seeding concentration
Identification of contamination through
Microscopic observation
Visual cues in media
Outcome
Compilation of culture observation summary
Interpretation of growth and health status of cells
Basic troubleshooting and reporting
Workshop Outcomes
By the end of this workshop, participants will:
Practice proper aseptic techniques
Prepare and handle cell culture media
Seed and maintain safe cell cultures
Perform cell viability assays and counting
Identify common contamination indicators
Document and summarize cell culture observations
Who Can Attend
Life sciences, biotechnology & biomedical students
Research interns and laboratory trainees
Faculty and technical staff
Beginners interested in learning cell culture techniques
📌 Limited Seats Available
Build essential cell culture skills with guided, hands-on laboratory training.
👉 Register now to secure your seat
📩 Click the Register button for schedule, fees, and certification details.