3-Day Hands-On Workshop on ELISA (Enzyme-Linked Immunosorbent Assay)
This workshop provides practical and conceptual training in ELISA principles, workflow, data analysis, and interpretation. It is designed for safe learning using mock samples and demonstrations, making it suitable for academic and training environments.
Day 1: ELISA Fundamentals & Plate Preparation
Concepts Covered
Introduction to immunoassays and ELISA formats
Direct, Indirect, Sandwich, and Competitive ELISA
Role of antibodies, antigens, and enzymes
Common sources of error in ELISA experiments
Hands-On / Demonstration
Microplate coating
Antigen or antibody coating (real or mock setup)
Preparation of wash buffers and blocking solutions
Understanding plate layout and controls
Skills Gained
Familiarity with ELISA workflow
Plate handling and pipetting accuracy
Buffer preparation and storage practices
Day 2: Antigen–Antibody Reaction & Detection
Concepts Covered
Antigen–antibody binding specificity
Signal generation and amplification
Importance of washing and incubation steps
Hands-On / Mock Experiments
Antigen–antibody reaction using mock or safe samples
Addition of detection antibody / enzyme conjugate
Color development step (demonstration-based)
Troubleshooting scenarios
High background
Weak signal
Inconsistent readings
Learning Outcome
Understanding critical control points in ELISA
Ability to identify and correct common assay issues
Day 3: OD Measurement, Data Analysis & Interpretation
Concepts Covered
Optical density (OD) measurement principles
Quantitative vs qualitative ELISA interpretation
Hands-On Training
OD reading using ELISA plate reader (or demo data)
Preparation of standard curve
Calculation of unknown sample concentration
Interpretation of positive, negative, and control results
Assessment & Outcome
Result validation and documentation
Certificate quiz to assess conceptual understanding
Discussion and wrap-up
Workshop Outcomes
By the end of this workshop, participants will:
Understand ELISA formats and workflow
Prepare plates, buffers, and controls
Analyze ELISA OD data and generate standard curves
Troubleshoot common ELISA problems
Interpret and report ELISA results confidently
Who Can Attend
Life science, biotechnology & biomedical students
Diagnostic laboratory trainees
Research interns and early-career researchers
Educators and laboratory professionals
📌 Limited Seats Available
Master the fundamentals of ELISA through guided, hands-on and demonstration-based learning.
👉 Register now to secure your seat
📩 Click the Register button for schedule, fees, certification, and quiz details.