3-Day Hands-On Workshop on Enzyme Activity, Kinetics & Inhibition
This workshop provides practical training in enzyme extraction, activity measurement, kinetic analysis, and inhibition studies. Participants will gain hands-on experience with real experimental data and mathematical modeling used in biochemical research and industry laboratories.
Day 1: Enzyme Fundamentals & Activity Assay
Concepts Covered
Introduction to enzymes and biological catalysis
Factors affecting enzyme activity: pH, temperature, substrate concentration
Types and sources of enzymes
Hands-On Experiments
Preparation of crude enzyme extract
Examples: Amylase (plant/animal source) or Catalase (tissue/microbial source)
Optimization of assay conditions
Enzyme activity assay using colorimetric / gas evolution methods
Skills Gained
Enzyme extraction techniques
Assay setup and reaction monitoring
Recording and interpreting enzyme activity data
Day 2: Enzyme Kinetics & Michaelis–Menten Analysis
Concepts Covered
Enzyme kinetics and reaction rates
Michaelis–Menten theory and assumptions
Significance of kinetic parameters
Hands-On Experiments
Kinetics experiment by varying substrate concentration
Measurement of initial reaction velocities
Construction of Michaelis–Menten curve
Data Analysis
Determination of Km and Vmax
Basic curve fitting and graphical interpretation
Understanding experimental variability
Day 3: Enzyme Inhibition & Model Interpretation
Concepts Covered
Enzyme inhibition mechanisms
Competitive inhibition
Non-competitive inhibition
Practical relevance in drug design and toxicology
Hands-On Experiments
Inhibition studies using safe inhibitors (e.g., metal ions)
Comparison of enzyme activity with and without inhibitors
Observation of changes in kinetic parameters
Data Analysis & Outcome
Model fitting of inhibited vs uninhibited reactions
Interpretation of kinetic plots
Drawing experimental conclusions
Compilation of results and discussion
Workshop Outcomes
By the end of this workshop, participants will:
Extract and assay enzymes confidently
Perform enzyme kinetic experiments
Calculate and interpret Km and Vmax
Differentiate between competitive and non-competitive inhibition
Analyze enzyme data using standard biochemical models
Who Can Attend
Life sciences, biotechnology & biochemistry students
Biomedical researchers
Laboratory professionals
Educators and trainees seeking hands-on enzyme analysis skills
📌 Limited Seats Available
Build strong fundamentals in enzyme kinetics through practical laboratory experience.
👉 Register now to participate in this hands-on workshop
📩 Click the Register button for dates, fees, and certification details.