3-Day Hands-On Workshop on Microbiology Techniques
This workshop introduces participants to fundamental microbiology laboratory practices, focusing on media preparation, aseptic handling, culturing techniques, staining, and microbial analysis. For safety and educational purposes, the workshop uses non-pathogenic, commonly encountered microorganisms.
Day 1: Media Preparation & Sterilization
Concepts Covered
Introduction to microbiology and microbial growth requirements
Types of culture media: simple, enriched, and selective (conceptual overview)
Principles of sterilization and contamination control
Hands-On Training
Preparation of culture media (e.g., nutrient agar)
Sterilization methods
Autoclave (if available)
Pressure cooker as an educational alternative
Pour plate technique
Safe handling of glassware and media
Skills Gained
Media preparation and pH adjustment
Understanding sterilization parameters
Basic aseptic technique
Day 2: Isolation Techniques Using Safe Cultures
Concepts Covered
Microbial isolation and pure culture principles
Importance of dilution and surface spreading
Hands-On Training
Streak plate technique
Spread plate technique
Culturing microorganisms from safe sources
Curd / yogurt
Soil microbes (educational sampling)
Incubation and observation of microbial growth
Learning Outcome
Isolation of distinct colonies
Comparison of streak vs spread plate outcomes
Maintaining contamination-free plates
Day 3: Staining, Enumeration & Microbial Analysis
Concepts Covered
Principles of bacterial staining
Colony morphology and its significance
Quantitative estimation of microbial load
Hands-On Training
Gram staining procedure and interpretation
Observation of colony characteristics
Size, shape, margin, elevation, and color
Colony Forming Unit (CFU) calculation
Recording and tabulating observations
Outcome
Preparation of a structured observation report
Correlation between staining results and colony morphology
Discussion and troubleshooting
Workshop Outcomes
By the end of this workshop, participants will:
Prepare and sterilize microbiological media
Perform basic microbial isolation techniques
Apply Gram staining and interpret results
Identify colony morphology characteristics
Calculate CFU accurately
Document microbiological observations systematically
Who Can Attend
Life science, biotechnology & microbiology students
Laboratory trainees and interns
Educators and academic institutions
Beginners interested in microbiology lab skills
📌 Limited Seats Available
Learn essential microbiology techniques through safe, hands-on laboratory training.
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